Figure 2. Human primary melanoma is enriched with NLRC4⁺ macrophages.

(A) Deidentified skin biopsies of normal skin (n = 3 patients), benign melanocytic nevi (n = 3 patients), primary invasive melanomas (n = 3 patients), and metastatic melanomas (n = 3 patients) were stained for CD163 (brown) and NLRC4 (brown). Images were taken with a ×40 objective. (B) The densities of five 20× high-power fields (each 3.14 mm²) were determined per patient sample. **P ≤ 0.01; ****P ≤ 0.0001, Mann–Whitney U test.