Skip to main content
. 2016 Sep 12;126(10):3917–3928. doi: 10.1172/JCI86953

Figure 6. NLRC4 regulates STAT3 and p38 MAPK signaling in the tumor microenvironment.

Figure 6

(A and B) B16F10 tumors from WT and Nlrc4–/– mice at day 14 after inoculation were homogenized and immunoblotted for phospho-STAT3 and STAT3 (A), phospho-p38 MAPK and p38 MAPK (B), and GAPDH (A and B). Each lane represents a tumor from an individual mouse. (A and B) Densitometry of the ratio of phosphorylated to total protein is shown. (C) WT and Nlrc4–/– BMDMs were challenged for 4, 5, 6, 7, and 8 hours with 50 ng/ml LPS. Cell lysates were immunoblotted with antibodies against phospho-STAT3, STAT3, and GAPDH. (D) WT and Nlrc4–/– BMDMs were challenged for 15, 30, 60, and 90 minutes with 50 ng/ml LPS. Cell lysates were immunoblotted with antibodies against phospho-p38 MAPK, p38 MAPK, and GAPDH. (E) WT and Nlrc4–/– BMDMs were challenged for 5, 10, 15, 30, and 60 minutes with 10 ng/ml recombinant IL-6. Cell lysates were immunoblotted with antibodies against phospho-STAT3, STAT3, and GAPDH. (C–E) Data are representative of 3 independent experiments. (A and B) *P ≤ 0.05, unpaired 2-tailed Student’s t test.