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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: J Neuromuscul Dis. 2016;3(3):395–404. doi: 10.3233/JND-160173

Fig. 3. MR protein levels are maintained in quadriceps muscles from het mice after 16 weeks of LS or EL treatment.

Fig. 3

Representative western blots of quadriceps muscles from 3 biological replicates are shown comparing MR protein levels from equivalent amounts (50 μg) of protein homogenates from: C57BL/10 wild-type mice (C57), dystrophin-deficient; utrophin haplo-insufficient mice (Het), lisinopril plus spironolactone treated Het mice (LS) and eplerenone plus lisinopril treated HET mice (EL). Western blots used a combination of MR-specific monoclonal antibodies MR1-18 1D5 & MRN 2B7 (full length MR predicted molecular weight ~107kDa, arrow) or a GAPDH antibody (loading control; predicted molecular weight ~36kDa). MR blots detected an additional lower molecular weight (~90 kDa) doublet, which are known degradation products resulting from snap freezing samples prior to processing [22].