Figure 4. Insr^T1150A mice fed a chronic HFD are protected from hepatic, but not skeletal muscle, insulin resistance.

Male Insr^T1150A and WT littermate mice were fed an HFD for 6 weeks and fasted overnight before hyperinsulinemic-euglycemic clamp studies. (A) Plasma glucose and glucose infusion rates during the clamp. (B) Mean steady-state glucose infusion rate. (C) EGP during the basal and steady-state periods of the clamp. (D) Suppression of EGP during the clamp, expressed as the percentage of basal EGP. (E and F) Hepatic AKT phosphorylation in post-clamp mice. (G) Whole-body glucose uptake during the steady-state period of the clamp. (H) ¹⁴C-2-deoxyglucose uptake during the clamp in gastrocnemius/soleus muscle. (A–H) Data represent the mean ± SEM . n = 5–7 mice per group. *P < 0.05, by unpaired, 2-tailed Student’s t test.