Figure 5. Increased insulin-stimulated hepatic glycogen synthesis in HFD-fed Insr^T1150A mice.

Male Insr^T1150A and WT littermate mice were fed an HFD for 8 to 10 days and fasted for 13 hours before undergoing hyperinsulinemic-hyperglycemic clamp studies. [U-¹³C]-glucose was infused to assess glycogen synthesis rates. (A) Total liver glycogen content after clamp studies. (B) Glycogen synthesis flux through the direct pathway during the clamp. (C) Total glycogen synthesis flux through both direct and indirect pathways during the clamp. (D) Relative Gck mRNA expression in fasted and post-clamp mice measured by qPCR and normalized to basal WT expression. (A–C) Data represent the mean ± SEM. n = 9 WT and n = 10 Insr^T1150A mice per group. *P < 0.05 and **P < 0.005, by unpaired, 2-tailed Student’s t test. (D) Data represent the mean ± SEM. n = 4 (basal) and n = 5–7 (post-clamp) mice per group. *P < 0.05 and ***P < 0.0005, by 2-way ANOVA with Holm-Sidak correction for multiple comparisons.