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. 2016 Oct 10;126(11):4174–4186. doi: 10.1172/JCI86623

Figure 1. Induction of EMT enables cancer cells to associate with blood vessels.

Figure 1

GFP-tagged DCIS-Snail-ER cells (green) were mixed with unlabeled DCIS epithelial cells (1:4) for orthotopic transplantation into immunodeficient mice. Tumor-bearing mice were treated with vehicle (A) or tamoxifen to induce EMT in DCIS-Snail-ER cells (B). Tumor vasculature was stained red with Griffonia simplicifolia lectin or antibodies against the endothelial markers CD31 (also known as PECAM1) and CD34. DNA was stained with Hoechst 33342 (blue). Scale bars: 50 μm.