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. 2016 Oct 4;126(11):4076–4087. doi: 10.1172/JCI88410

Figure 3. PD blocks IR-induced crypt apoptosis.

Figure 3

Mice were pretreated with vehicle, PD, or LEE and subjected to 15 Gy TBI. The small intestine was analyzed at the indicated time points. (A) Representative images of TUNEL and cleaved caspase-3 (CASP-3) staining of intestinal sections at 4 hours. Scale bars: 20 μm. (B) Quantitation of TUNEL+ and cleaved caspase-3+ crypt cells at 4 and 24 hours. (C) Fractions of cleaved caspase-3+ crypt cells at 4 hours based on cell positions. (D) Quantification of TUNEL and BrdU index at 4 hours and regenerated crypts at 96 hours in LEE-treated and control (Ctrl) mice. (E) Intestinal expression of the indicated proteins analyzed by Western blotting. β-Actin was used as the loading control. Lysates were pooled from 3 mice per group. Replicate samples run on separate gels are presented. (F) Intestinal relative expression of the indicated mRNAs versus the nontreated mice. cDNA was synthesized from RNA pooled from 3 mice per group. Similar results were obtained in at least 3 independent experiments for Western blotting and RT-PCR. (B–D and F) Values represent the mean ± SEM; N = 3 mice in each group. *P < 0.05, **P < 0.01, and ***P < 0.001, vehicle versus PD or LEE treatment, by unpaired, 2-tailed Student’s t test.