Figure 4. Calcium mobilization is inhibited in desensitized cells.

(A) IgE-sensitized BMMCs were left untreated (blue) or desensitized (red). After labeling with Fluo-4, cells were challenged with Ag (arrow) for 5 minutes. Responsiveness was confirmed by addition of ionomycin. Fluorescence was normalized to baseline. A representative graph is shown. AUC analyses were averaged from at least 4 independent experiments. (B) 5 × 10⁵ BMMCs per sample in triplicate were desensitized or control treated before addition of 1 μg/ml ionomycin. Degranulation was measured via β-hexosaminidase assay. Data were analyzed via 2-tailed Student’s t test. Error bars represent SEM. **P < 0.01.