Figure 7. Antigen specificity of desensitization.

(A) RBL-2H3 cells were sensitized with a 1:5 ratio of anti-DNP to anti-OVA IgE, then desensitized to DNP-HSA or OVA before challenge with DNP-HSA (10 ng/ml) or OVA (1 μg/ml). Data were analyzed via 1-way ANOVA and are representative of at least 3 independent experiments. (B and C) 10⁷ BMMCs per sample were sensitized with biotinylated IgE, then challenged with Ag for the indicated times. Lysates were immunoprecipitated with streptavidin beads and blotted for β-actin and FcεRIγ. (D) 10⁷ BMMCs per sample were sensitized with a mixture of biotinylated and unbiotinylated IgE, then desensitized or challenged with Ag for the same amount of time (~2 hours). Lysates were then immunoprecipitated with streptavidin beads and blotted for β-actin and FcεRIγ. Data represent SEM. ***P < 0.001.