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. 2016 Oct 17;126(11):4205–4218. doi: 10.1172/JCI87927

Figure 6. Tug1 enhances Ppargc1a promoter activity via the TBE.

Figure 6

(A) Schematic of Ppargc1a promoter-luciferase constructs. Right, luciferase reporter activity in control and Tug1-KD podocytes. Inclusion of the TBE leads to robust enhancement of promoter activity, which is significantly attenuated in Tug1-KD cells. **P < 0.01, and ***P < 0.001, by 2-tailed Student’s t test. luc, luciferase; F, forward; R, reverse. (B) Schematic of the genomic context of the TBE. The highlighted sequences refer to the sense strand of the TBE. The GA-repeat motif is located in the antisense strand. Genomic coordinates refer to Ensembl mouse genome assembly GRCm38. Red arrows indicate the position of the sgRNAs designed to target the TBE element. gRNAs, guide RNAs. (C) qPCR analysis of Ppargc1a mRNA in control podocytes and 3 independent TBE targeted clones. Expression values normalized to Gapdh internal controls. Cell culture experiments repeated at least 3 times. Data are expressed as the mean ± SEM. Ctrl, control.