3T3-L1 cells were pretreated with Rimonabant (0.1, 0.2, or 0.5 μM) or vehicle (DMSO) for two hours. Then, the cells were induced to differentiate in the presence of AEA (10 μM), butylparaben (50 μM) or benzylparaben (50 μM), while maintained in rimonabant for 7 days. (A) Increasing concentrations of rimonabant had no effect on paraben-enhanced adipocyte differentiation as measured by oil red O. (B) Effects of rimonabant on parabens-mediated upregulation of adipocyte marker PPARγ, C/EBPα, and FABP4. The dark triangles indicate the increasing doses of rimonabant shown in (A). *p < 0.05 compared with the control (0 μM of rimonabant) within each treatment group. NS, not significant.