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. Author manuscript; available in PMC: 2017 Nov 3.
Published in final edited form as: Mol Cell. 2016 Oct 20;64(3):443–454. doi: 10.1016/j.molcel.2016.09.015

Figure 1. A single Mediator complex bridges the enhancer and core promoter.

Figure 1

(A) Med14 occupancy upstream of the CCW12 and DLD3 transcription start site (dashed red line) in a kin28-AA strain in the presence or absence of rapamycin. Med14 associates with the CCW12 enhancer but not the DLD3 enhancer, but it associates with both promoters when Kin28 is depleted.

(B) Sequential ChIP at the SPO24 and CCW12 enhancer and promoter regions. Kin28 anchor-away strains contain either two copies of MED14 separately tagged with 6x-HA and 13x-MYC (left panel) or MED15 tagged with 6x-HA and RGR1 with 13x-MYC (right panel) and were grown in the presence or absence of rapamycin. ChIP was performed with α-HA, followed by α-Myc antibodies (light blue), or vice versa (purple), as well as with HA or Myc alone (gray and yellow, respectively). Fold-enrichments of the indicated genomic regions (red lines) with respect to the control region are shown.

See also Figure S1.