A) Occupancy of the indicated Mediator subunits at the CCW12 and HSP82 enhancers of the indicated genes in a med17-AA strain that was or was not treated for 1 hr with rapamycin and was or was not subjected to a 15 min heat shock at 39°C. Immunoprecipitations were performed with subunit-specific antibodies (a kind gift from the Hahn lab), except for Med15 and Cdk8, which were tagged with 13x-MYC.
(B) Similar experiment except performed in a strain that permits simultaneous depletion of Srb6 and Med7.
(C) Med3 Occupancy at the CCW12 and SED1 enhancer and core promoter before or after Kin28 depletion (blue) or simultaneous depletion of Kin28 and Med17 (red).
See also Figures S2 and S3.