Figure 9.

Slow turnover of cyclin E2 protein accounts for its failure to correlate with mRNA levels. (a) The lentiviral p53 knockdown cells (LVp53KO) and control cell lines were grown in RPMI media containing 0 or 10 μm BeSO ₄ for 2 d, then either 0 or 10 μm MG‐132 (a proteasome inhibitor) was added for 6 h, and lysates were analysed by Western blotting. (b) A172 or A172‐E6 cells were grown in 0 or 10 μm BeSO ₄ for 2 d and 10 μm MG‐132 for 0, 3 or 6 h, and analysed by Western blotting. (c) Normal A172 cells were grown in RPMI media containing 0 or 10 μm BeSO ₄ for 2 d, followed by fresh media containing 100 μg/mL cycloheximide (a protein synthesis inhibitor) for 0, 4, 8 or 12 h, and lysates were analysed by Western blotting