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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Pharm Res. 2016 Sep 12;33(12):2954–2966. doi: 10.1007/s11095-016-2017-y

Figure 1.

Figure 1

Conjugation of scFv7F9Cys to a linear 5 kDa (lane 2), linear 20 kDa (lane 3), and branched 40 kDa PEG (lane 4). ScFv7F9Cys was incubated with a 20-fold excess of PEG. Each PEG used contained a maleimide functional group that interacted with the free sulfhydryl group of scFv7F9Cys to form a permanent scFv7F9Cys-PEG20K product.