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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Pharm Res. 2016 Sep 12;33(12):2954–2966. doi: 10.1007/s11095-016-2017-y

Figure 2.

Figure 2

IMAC purification of scFv7F9Cys–PEG20K. A nickel affinity column was used. ScFv7F9Cys contains a poly-histidine tag which will bind the nickel on the column. Any bound protein was eluted using imidazole increasing in concentration over 6 steps (0, 5, 10, 15, 20, and 100%). A. Chromatogram of purification: solid line shows UV280 peaks, and dashed line shows the percent of imidazole. B. SDS-PAGE analysis of fractions from IMAC purification. Bars 1, 2, and 3, correspond to fractions analyzed from same section of the chromatogram. C. SDS-PAGE analysis of scFv7F9Cys-PEG40K IMAC purification.