Figure 6. SWR and SWR-associated slow gamma characteristics in young apoE3-KI and apoE4-KI mice are differentially affected by aging.
(A) SWR abundance in 4–5-month-old apoE3-KI and apoE4-KI mice at 3, 4, 5, and 6 SD thresholds. (unpaired t tests; t(14) = 3.129, 3.126, 2.939, and 2.634, respectively).
(B) Percent power spectra during SWRs. Frequency of peak ripple power is significantly lower in young apoE4-KI mice than in young apoE3-KI mice; 156.0 ± 1.562 Hz vs 175.1 ± 1.98 Hz, (unpaired t test, P < 0.0001; t(14) = 7.252).
(C) Quantification of average SWR length in young apoE3-KI and young apoE4-KI mice (unpaired t-test, t(14) = 3.431)
(D) Quantification of SWR-associated slow gamma power (z-scored) in hippocampal subregions (unpaired t test; t(14) = 3.281 for CA1-sr).
(E) Distribution of SWR-associated slow gamma power (z-scored) in CA1-sr. Inset: Recalculation of average SWR-associated slow gamma power excluding events with z-scored slow gamma power > 7 (dashed line).
(F) Distribution of SWR-associated slow gamma power (z-scored) in CA1-sr for all genotype and age groups.
(G) SWR-associated slow gamma power in CA1 stratum radiatum recalculated after exclusion of events with z-scored slow gamma power > 7 (dashed line in panel F). Two-way ANOVA with corrected post-hoc tests shows significant effects of genotype (P < 0.0001, F(1,39) = 23.00) and age (P = 0.0388, F(1,39) =4.571) and significant interaction between the two (P = 0.0263, F(1,39) = 5.332).
(H) Fraction of SWRs with suppressed CA1 stratum radiatum slow gamma activity below baseline (z-score power < 0) for all genotype and age groups. Two way ANOVA with corrected post-hoc tests shows significant effects of genotype (P < 0.0001, F(1,39) = 32.84) and age (P = 0.0013, F(1,39) = 11.92) and significant interaction between the two (P = 0.0033, F(1,39) = 9.823).
*P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. n = 9 and 7 for young apoE3-KI and apoE4-KI, respectively; n = 14 and 13 for aged apoE3-KI and apoE4-KI, respectively. Error bars and shading indicate SEM. See also Figure S6.