FIG. 3.

FITC Dextran flowed through the device (without tissue in Panel A) using a PDMS plug and petroleum jelly to ensure a thorough seal (Panel A, green line) and without petroleum jelly to determine leakage (Panel A, blue line). Panel A: Shows the FITC flowed through the device ensuring FITC remains on the luminal side of the device only. Panel B: A frozen section of tissue used to show fluorescence passing through the tissue. Panel C: Dark field image of tissue to determine structure without FITC.