Figure 3.

Activation of the UPR-induced apoptotic machinery. AMO-1 derivatives adapted to bortezomib (AMO-BTZ) and carfilzomib (AMO-CFZ) have been cultured for 2 weeks without proteasome inhibitors. Then, together with AMO-1 sensitive cell line, they were treated with 500 nm bortezomib for a 1-h pulse. After the pulse, cells were grown in fresh medium for different chase time points (0.5, 1, 2, 4, 8, 16, 24 h). Directly before the addition of the proteasome inhibitor (presample) and immediately after the pulse (postsample), as well as at each chase time point indicated, an aliquot was harvested for protein isolation, and equal amounts of protein from each sample were subsequently processed for western blotting. Data are presented as representative bands of three independent experiments.