Table 2. Proteomic comparison of AMO-1 cells with AMO-BTZ and AMO-CFZ.
| Change | GO term | P-value range | Number of proteins | Mean fold change (range) |
|---|---|---|---|---|
| AMO-BTZ | ||||
| Upregulated | Protein catabolism | 10−8 | 19 | 1.7 (2.3–1.4) |
| Redox homeostasis | 10−7–10−6 | 25 | 1.9 (6.1–1.4) | |
| Folding and complex formation | 10−3–10−2 | 53 | 2.0 (19.7–1.4) | |
| Transport/signaling | 10−3–10−2 | 116 | 1.7 (4.3–1.4) | |
| Metabolic regulation | 10−3–10−2 | 114 | 1.8 (9.2–1.4) | |
| Downregulated | Transcription/translation | 10−2 | 63 | 0.6 (0.7–0.2) |
| Differentiation | 10−2 | 13 | 0.6 (0.7–0.4) | |
| Cytoskeleton organization | 10−2 | 22 | 0.5 (0.7–0.1) | |
| Metabolic regulation | 10−2 | 45 | 0.6 (0.7–0.4) | |
| Transport/signaling | 10−2 | 89 | 0.5 (0.7–0.1) | |
| Apoptosis | 10−1 | 8 | 0.5 (0.7–0.3) | |
| AMO-CFZ | ||||
| Upregulated | Protein catabolism | 10−8 | 22 | 1.7 (2.7–1.4) |
| Transport/signaling | 10−6–10−2 | 85 | 1.9 (12.1–1.4) | |
| Metabolic regulation | 10−6–10−2 | 110 | 1.8 (4.6–1.4) | |
| Folding and complex formation | 10−3–10−2 | 39 | 2.2 (13.9–1.4) | |
| Redox homeostasis | 10−3–10−2 | 15 | 2.2 (4.3–1.4) | |
| Downregulated | Apoptosis | 10−3 | 7 | 0.5 (0.7–0.2) |
| Metabolic regulation | 10−3–10−2 | 58 | 0.5 (0.7–0.2) | |
| Transport/signaling | 10−2 | 72 | 0.5 (0.7–0.1) | |
| Transcription/translation | 10−2 | 58 | 0.6 (0.7–0.2) | |
| Differentiation | 10−2 | 10 | 0.5 (0.7–0.2) | |
| Cytoskeleton organization | 10−2 | 24 | 0.5 (0.7–0.1) | |
| HL60–BTZ | ||||
| Upregulated | Protein catabolism | 10−15 | 23 | 1.7 (2.7–1.4) |
| Transport/signaling | 10−10–10−2 | 80 | 1.8 (6.8–1.4) | |
| Metabolic regulation | 10−3–10−2 | 65 | 1.8 (4.5–1.4) | |
| Folding and complex formation | 10−2 | 28 | 1.8 (3.9–1.4) | |
| Redox homeostasis | 10−2 | 20 | 1.9 (4.4–1.4) | |
| Downregulated | Transport/signaling | 10−5 | 40 | 0.6 (0.7–0.3) |
| Metabolic regulation | 10−3–10−2 | 34 | 0.6 (0.7–0.3) | |
| Transcription/translation | 10−2 | 64 | 0.6 (0.7–0.2) | |
| Redox homeostasis | 10−2 | 16 | 0.6 (0.7–0.2) | |
| Cytoskeleton organization | 10−2 | 12 | 0.6 (0.7–0.4) | |
Abbreviation: GO, gene ontology. Supervised clustering of GO terms into functionally related clusters of biological processes found for the upregulated and downregulated proteins in the cell lines adapted to bortezomib (AMO-BTZ, HL60-BTZ) and carfilzomib (AMO-CFZ). P-values range is obtained from the unsupervised clusters of different GO terms (see Supplementary Table S1) clustered in each category.