Fig. 1.

The majority of cytosolic Ku70 is found in high molecule weight complexes. a Cytosolic SH-SY5Y cell extracts were analyzed by Superdex 600 HR 10/30 gel filtration chromatography. Twenty microliter of each fraction (0.5 ml) was separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and the blot was probed with Ku70, Bax, or Ku80 antibodies. b Cytosolic extracts were cross-linked using DC4 (0, 0.1, 0.2, 0.4, 0.8, 1.6 mM) as shown. Cross-linked proteins were separated by 10 % SDS-PAGE, and the blot was probed with Ku70, Ku80, or Bax antibodies