Fig. 2.

No interaction between Bax and Ku80. a SH-SY5Y cells were treated with suberoylanilide hydroxamic acid (SAHA) (4 μM) for 48 h. Control cells received the same volume of DMSO. Cytosolic extracts were immunoprecipitated using an anti-Bax antibody. Normal rabbit serum (NRS) was used as a negative control. The immunoprecipitated complex was separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and the blot was probed with Ku70, Ku80, or Bax antibodies. b SH-SY5Y cells were treated with SAHA (4 μM) for 48 h. Control cells received the same volume of DMSO. Cytosolic extracts were immunoprecipitated using Ku70 or Ku80 antibodies. Normal mouse serum for Ku70 immunoprecipitation or normal rabbit serum for Ku80 immunoprecipitation was used as an immunoprecipitation control, respectively. The immunoprecipitated complex was separated by SDS-PAGE, and the blot was probed with Ku70 or Ku80 antibodies