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List of primer used for qPCR
| Primer name | Sequence (5′–3′) | Target genes | qPCR efficiency (%) | Amplification factor |
|---|---|---|---|---|
| MTC_forward MTC_reverse |
AGCCAATATTTTCGAGTGGAGA CAAGATGCTCGAATAGCAACAGTA |
Metallothionein-like containing protein [20] | 87.50 | 1.87 |
| GST_forward GST_reverse |
CATCAACATTGCCGACAAAC ACGACAGCCTTGAACTTTGC |
Glutathione S-transferase [22] | 96.53 | 1.97 |
| YWHAZ_forward YWHAZ_reverse |
TCGCCCTCAACTTTTCCGTT TGCTATCGCTTCATCGAATGC |
Tyrosine 3-monooxygenase [23] | 88.44 | 1.88 |
