Figure 4. VH298 elicits a HIF-dependent hypoxic response in various cell lines.

1% DMSO, hypoxia (1% O₂) and indicated compounds at 100 μM were introduced to (a) HFF, HeLa, U2OS and (b) RCC4 cells expressing HA-tagged VHL (with the exception of cis-VH298 and VH298 used at 150 μM) for 24 h and (c) CTLs for 8 h. Protein levels were analysed by immunoblotting using antibodies against the indicated proteins, with β-actin (SMC1 for CTLs) as the loading control. The blots shown are representative of three independent experiments.