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. 2016 Nov 4;7:13287. doi: 10.1038/ncomms13287

Figure 8. Specific interactome is essential for GAS5 to mediate NODAL signalling.

Figure 8

(a,b) The analysis of the expression level of NODAL, GAS5 (b) and their interacting miRNAs (a) in different cell types. **P<0.01, t-test, n=3. (c) The relative mRNA level of pluripotency genes and NODAL in the GAS5 overexpressed groups were compared with different cell types using qPCR. The expression levels of pluripotency genes and NODAL in each cell type is compared with their relative empty vector expressed group, which were shown as a dashed line. **P<0.01, t-test, n=3. (d,e) ELISA analysis of the secreted NODAL in the cell culture medium of different cell types (d) and GAS5 overexpressed or knockdown cells (e). All cells for supernatant collecting were cultured using serum-free medium. **P<0.01, t-test, n=3. (f–h) Standardized quantitative PCR detecting the expression change of indicated miRNA and NODAL transcript in hESCs (f), uMSCs (g) and HEK-293T cells (h) under different concentration of synthetic full-length GAS5 RNA transfection. n=3. (i) The graphical summary of the function and mechanism of GAS5. Error bars represent s.d. of the indicated experiment replicates. RNA level of β-actin served as internal reference for qPCR. See also Supplementary Fig. 8.