Figure 7. IFNγ-dependent chemokine expression and emergence of CD45^high haematopoietic cells.

CCL2, CCL3, CCL4, CCL5, CXCL9, CXCL10 and IFNγ mRNA expression was analysed in brain tissue of uninfected or PbA-infected (a) IFNγ^−/− and C57BL/6 mice, as well as (b) C57BL/6 mice treated from day −1 to 3 post infection with vehicle or E6446 (120 mg kg⁻¹ per day), as indicated. The mRNA levels were analysed by quantitative PCR and results presented after normalization with β2-microglobulin mRNA. (c) Flow cytometric analysis of brain MO-DCs first selected on CD45high, gated on CD3⁻CD8⁻ and then CD11b⁺Ly6c⁺ (CD11c⁺DC-SIGN⁺) from uninfected and PBA-infected WT, IFNγ^−/− and WT mice treated with E6446 7 days post infection. The numbers of both CD45^highCD8⁺CD3⁺ and CD45^highCD8⁻CD3⁻CD11c⁺CD11b⁺DC-SIGN^highLy6c⁺ cells are significantly decreased in the brain of infected IFNγ^−/− and E6446-treated mice. Differences considered statistically significant when *P<0.05, **P<0.01, ***P<0.001 and ****P<0.0001 as indicated by two-way analysis of variance analysis (values are means±s.d.).