Figure 8. Effect of caspofungin on poly-β(1-6)-N-acetylglucosamine in biofilms.
Biofilms were incubated during 24 h in the absence (control) or in the presence (+CAS) of 40 mg l−1 caspofungin. (a) Immunoblot analysis of PNAG purified from biofilms. (b) Determination of N-acetyl-glucosamine (GlcNAc) concentration after treatment of purified PNAG during 1 h with 0.1% Dispersin B. The released GlcNAc monomers were detected by fluorescence (λexc 545 nm; λem 604 nm). All data are mean±s.d. of triplicates. Statistical analysis (two-way ANOVA; Tukey post-hoc test): groups with different letters (caps: control; small letters: +CAS) are significantly different from one another (P<0.05); *** (P<0.001): comparison of control to CAS for each individual strain.