Fig. 1.

Overview of the Q-nexus preprocessing workflow. During barcode preprocessing, barcode tags are removed. Subsequently, adapter sequences are clipped and reads that consist completely of adapter (orange-tagged) are removed. The clipped reads are mapped to the reference genome. The random barcode tags allow PCR duplicated reads and IMUB reads to be distinguished from one another. Only one of the two PCR duplicated reads mapping to the same genomic position (blue-tagged) is kept, while reads with different random barcodes are allowed to map to the same position