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. 2016 Nov 4;17:873. doi: 10.1186/s12864-016-3164-6

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© The Author(s) 2016

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver(http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Duplication levels using random barcodes. a Toy example of mapped ChIP-nexus reads and the corresponding counts for identically mapped reads (IM, red), identically mapped reads with identical random barcodes (IMIB, blue), and identically mapped reads with unique random barcodes (IMUB, black). The number of horizontal bars for a given level corresponds to the number of reads that have the same level of duplication. Additional file 1: Figure S1 provides a detailed example of how IM, IMIB, and IMUB reads are defined and calculated. b, c Duplication level plots for a Dorsal dataset with an overall duplication level of 54 % and for a Max dataset with an overall duplication level of 95 %