Table 5.
SPOT score for ChIP-seq datasets from GM12878 (A) and mouse ES cells (B)
| Epitope | Dataset | SPOT score (%) |
|---|---|---|
| (A) SPOT scores for GM12878 monoclonal and polyclonal datasets | ||
| H3K27ac | Monoclonal Rep 1 | 37.42 |
| Monoclonal Rep 2 | 38.86 | |
| Polyclonal ENCFF000ASP | 45.34 | |
| Polyclonal ENCFF000ASU | 19.41 | |
| H3K27me3 | Monoclonal Rep 1 | 45.67 |
| Monoclonal Rep 2 | 47.53 | |
| Polyclonal ENCFF000ASV | 40.92 | |
| Polyclonal ENCFF000ASW | 48.89 | |
| Polyclonal ENCFF000ASZ | 24.35 | |
| H3K4me1 | Monoclonal Rep 1 | 27.84 |
| Monoclonal Rep 2 | 25.66 | |
| Polyclonal ENCFF000ASM | 45.47 | |
| Polyclonal ENCFF000ATK | 33.47 | |
| H3K4me3 | Monoclonal Lot 8 Rep 1 | 65.41 |
| Monoclonal Lot 8 Rep 2 | 67.41 | |
| Monoclonal Lot 9 Rep 1 | 62.18 | |
| Monoclonal Lot 9 Rep 2 | 58.64 | |
| Polyclonal ENCFF000ASR | 27.53 | |
| Polyclonal ENCFF000AUB | 16.47 | |
| H3K9me3 | Monoclonal K9me3 Rep 1 | 25.24 |
| Monoclonal K9me3 Rep 2 | 24.76 | |
| Polyclonal ENCFF000AUK | 25.50 | |
| Polyclonal ENCFF000AUO | 28.59 | |
| (B) SPOT scores for mouse ES cells monoclonal and polyclonal datasets | ||
| H2K27ac | Monoclonal Rep1 | 11 |
| Monoclonal Rep2 | 11 | |
| Polyclonal Mouse ENCODE Bruce4 Rep 1 | 16 | |
| Polyclonal Mouse ENCODE Bruce4 Rep 2 | 14 | |
| Polyclonal Mouse ENCODE E14 Rep 1 | 6 | |
| Polyclonal Mouse ENCODE E14 Rep 2 | 16 | |
| H3K27me3 | Monoclonal Lot 1 Rep1 | 3 |
| Monoclonal Lot 1 Rep2 | 3 | |
| Polyclonal Mouse ENCODE Bruce4 Rep 1 | 4 | |
| Polyclonal Mouse ENCODE Bruce4 Rep 2 | 4 | |
| H3K4me1 | Monoclonal Rep 1 | 16 |
| Monoclonal Rep 2 | 15 | |
| Polyclonal Mouse ENCODE Bruce4 | 30 | |
| Polyclonal Mouse ENCODE E14 Rep 1 | 24 | |
| Polyclonal Mouse ENCODE E14 Rep 2 | 27 | |
| H3K4me3 | Monoclonal Lot 8 Rep 1 | 33 |
| Monoclonal Lot 8 Rep 2 | 27 | |
| Monoclonal Lot 9 Rep 1 | 30 | |
| Monoclonal Lot 9 Rep 2 | 27 | |
| Polyclonal Mouse ENCODE Bruce4 Rep 1 | 36 | |
| Polyclonal Mouse ENCODE Bruce4 Rep 2 | 37 | |
| Polyclonal Mouse ENCODE E14 Rep 1 | 42 | |
| Polyclonal Mouse ENCODE E14 Rep 2 | 39 | |
| H3K9me3 | Monoclonal Rep1 | 3 |
| Monoclonal Rep2 | 3 | |
| Polyclonal Mouse ENCODE Bruce4 Rep 1 | 11 | |
| Polyclonal Mouse ENCODE Bruce4 Rep 2 | 12 | |
SPOT score is calculated as the percent of reads that overlap with peaks. For each antibody, peak calls generated by the Mouse ENCODE for the Bruce4 ES cell line (polyclonal antibody) were used to define the peak coordinates