Skip to main content
. Author manuscript; available in PMC: 2017 Jan 14.
Published in final edited form as: Gene Ther. 2016 Jul 14;23(11):767–774. doi: 10.1038/gt.2016.54

Figure 5.

Figure 5

GFP fluorescence patterns of different rAAV serotypes following intravitreal injection in wild type (WT) C57BL/6 and Pde6brd1/rd1 (rd1) mice. The main images a-f are confocal stacks illustrating overall GFP expression patterns (green). The boxed regions are enlarged and shown as confocal slices in panels bi-iii and ei-iii. Images show GFP expression (green), nuclear labeling (blue) and immunostaining with anti-PKCalpha antibody identifying bipolar cells (red). Colocalisation of red and green signals therefore indicates instances of viral transduction of bipolar cells. GCL= ganglion cell layer, INL= inner nuclear layer, ONL=outer nuclear layer, RPE= retinal pigment epithelium. Scale bar 50 μm for images a-c, 30μm for images d-f.