Figure 5.

Non-metric 2-dimensional scaling analysis (NMDS) revealing distinct clustering of transconjugal pools (circles) and recipient communities (triangles). Three transconjugal clusters are identified and named with roman numerals. Experimental treatments (type of metal and degree of stress as operationally defined by % inhibition of [³H]leucine incorporation rates) are shown by color and size of symbols. Amplicon pools were subsampled at 50 000 sequences. OTUs from the recipient pool that were not represented in any transconjugal pool were removed prior to subsampling and ordination based on weighted OTU abundance using the Theta Yue Clayton (Yue and Clayton, 2005) algorithm.