Figure 1.
Tumour CD274 expression in colorectal cancer. Tumour CD274 expression was evaluated based on immunostaining in the cytoplasm and membrane of tumour cells. Cytoplasmic expression level was scored as 0 (A), 1 (B), 2 (C), or 3 (D), according to cytoplasmic intensity. (E) Membrane CD274 expression in tumour cells. Membrane expression level was scored as 0 (absent) or 1 (present; if distinct membrane staining above cytoplasmic staining level existed). (F) Immune cells, including lymphocytes and macrophages, in the lymphoid tissue within normal colorectal tissue cores served as positive controls.