Table 2.
Distribution of colorectal cancer cases according to the tumour CD274 expression score, the densities of T cells, and histological lymphocytic reaction patterns
| Total No. | Tumour CD274 expression score
|
P value* | |||||
|---|---|---|---|---|---|---|---|
| 0 | 1 | 2 | 3 | 4 | |||
| CD3+ cell density (n=571) | 0.39 | ||||||
| Quartile 1 (lowest) | 143 (25%) | 16 (29%) | 48 (31%) | 32 (22%) | 41 (23%) | 6 (17%) | |
| Quartile 2 (second) | 142 (25%) | 12 (21%) | 37 (24%) | 29 (20%) | 52 (29%) | 12 (33%) | |
| Quartile 3 (third) | 143 (25%) | 11 (20%) | 38 (24%) | 37 (25%) | 47 (27%) | 10 (28%) | |
| Quartile 4 (highest) | 143 (25%) | 17 (30%) | 33 (21%) | 48 (33%) | 37 (21%) | 8 (22%) | |
| CD8+ cell density (n=564) | 0.13 | ||||||
| Quartile 1 (lowest) | 141 (25%) | 16 (30%) | 44 (29%) | 29 (20%) | 42 (24%) | 10 (29%) | |
| Quartile 2 (second) | 141 (25%) | 10 (19%) | 42 (27%) | 41 (28%) | 43 (24%) | 5 (15%) | |
| Quartile 3 (third) | 141 (25%) | 15 (28%) | 39 (25%) | 32 (22%) | 48 (27%) | 7 (21%) | |
| Quartile 4 (highest) | 141 (25%) | 13 (24%) | 29 (19%) | 44 (30%) | 43 (24%) | 12 (35%) | |
| CD45RO+ cell density (n=577) | 0.031 | ||||||
| Quartile 1 (lowest) | 145 (25%) | 22 (39%) | 42 (26%) | 31 (21%) | 42 (24%) | 8 (23%) | |
| Quartile 2 (second) | 144 (25%) | 9 (16%) | 47 (29%) | 42 (29%) | 38 (21%) | 8 (23%) | |
| Quartile 3 (third) | 143 (25%) | 12 (21%) | 42 (26%) | 31 (21%) | 49 (28%) | 9 (26%) | |
| Quartile 4 (highest) | 145 (25%) | 13 (23%) | 30 (19%) | 43 (29%) | 49 (28%) | 10 (29%) | |
| FOXP3+ cell density (n=549) | <0.0001 | ||||||
| Quartile 1 (lowest) | 137 (25%) | 8 (15%) | 35 (23%) | 33 (24%) | 45 (26%) | 16 (44%) | |
| Quartile 2 (second) | 137 (25%) | 9 (16%) | 34 (23%) | 35 (26%) | 52 (31%) | 7 (19%) | |
| Quartile 3 (third) | 138 (25%) | 13 (24%) | 44 (29%) | 33 (24%) | 42 (25%) | 6 (17%) | |
| Quartile 4 (highest) | 137 (25%) | 25 (45%) | 38 (25%) | 36 (26%) | 31 (18%) | 7 (19%) | |
| Crohn’s-like lymphoid reaction (n=681) | 0.08 | ||||||
| Absent | 509 (75%) | 46 (68%) | 147 (75%) | 136 (71%) | 155 (80%) | 25 (78%) | |
| Low | 126 (19%) | 14 (21%) | 33 (17%) | 44 (23%) | 31 (16%) | 4 (13%) | |
| High | 46 (7%) | 8 (12%) | 15 (8%) | 12 (6%) | 8 (4%) | 3 (9%) | |
| Peritumoural lymphocytic reaction (n=808) | 0.36 | ||||||
| Absent | 122 (15%) | 7 (8%) | 37 (16%) | 44 (21%) | 28 (12%) | 6 (15%) | |
| Low | 562 (70%) | 63 (72%) | 155 (68%) | 137 (64%) | 180 (76%) | 27 (66%) | |
| High | 124 (15%) | 18 (20%) | 36 (16%) | 33 (15%) | 29 (12%) | 8 (20%) | |
| Intratumoural periglandular reaction (n=811) | 0.93 | ||||||
| Absent | 101 (12%) | 7 (8%) | 32 (14%) | 38 (18%) | 20 (8%) | 4 (10%) | |
| Low | 614 (76%) | 70 (79%) | 170 (74%) | 148 (69%) | 194 (82%) | 32 (78%) | |
| High | 97 (12%) | 12 (13%) | 29 (13%) | 28 (13%) | 23 (10%) | 5 (12%) | |
| Tumour-infiltrating lymphocytes (TIL) (n=811) | 0.006 | ||||||
| Absent | 592 (73%) | 55 (62%) | 163 (71%) | 157 (73%) | 187 (79%) | 30 (73%) | |
| Low | 130 (16%) | 21 (24%) | 42 (18%) | 32 (14%) | 31 (13%) | 4 (10%) | |
| High | 89 (11%) | 13 (15%) | 26 (11%) | 25 (12%) | 18 (8%) | 7 (17%) | |
*
P value was calculated by Spearman correlation test between the tumour CD274 expression score (ranging from 0 to 4) and (T) lymphocyte variables, including the densities of T cells (cells/mm2; as continuous variables) and histological lymphocytic reaction patterns (absent, low, and high; as ordinal variables). Because we assessed the eight primary (T) lymphocyte variables, we adjusted two-sided α level to 0.006 (=0.05/8) by simple Bonferroni correction.