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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: J Periodontal Res. 2016 May 5;52(2):246–254. doi: 10.1111/jre.12388

Fig. 1. A–D: P. gingivalis–induced neutrophil cytokine and chemokine responses.

Fig. 1

5×105 neutrophils, from either healthy controls (n=10) or subjects with localized aggressive periodontitis (LAgP) (n=10), suspended in RPMI-medium containing 20% (v/v) AB serum, were stimulated with 1×107 P. gingivalis for 1 hour at 37°C in presence (■) or absence (○) of 3.6×107 autologous red blood cells (RBCs). Culture supernatants were thereafter assessed for content of A) TNF-α, B) IL-6, C) CCL2 and D) CXCL8. The presence of RBCs bound to neutrophils significantly increased release of TNFα, IL-6 and CCL2. There were no differences in the response of LAgP and healthy control neutrophils under any conditions. The lower limits of detection provided by the manufacturer were A) 0.16, B) 0.11, C) 1.9 and D) 0.13 as indicated with a dotted line. ***p<0.0001.