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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: J Periodontal Res. 2016 May 5;52(2):246–254. doi: 10.1111/jre.12388

Fig. 2. P. gingivalis–induced generation of reactive oxygen species (ROS) in neutrophils.

Fig. 2

Dihydroethidium (DHE)-labeled 5×105 neutrophils (PMN), from either healthy controls (n=6) (○) or subjects with localized aggressive periodontitis (LAgP) (n=6) (■) were suspended in media containing 20% (v/v) AB serum and stimulated with 1×107 Porphyromonas gingivalis (P.g.) for 1 hour at 37°C. ROS is expressed as median fluorescence intensity of individual donors, in i) unstimulated, non-labelled neutrophils, ii) unstimulated DHE-labeled neutrophils and iii) DHE-labeled neutrophils stimulated with P.g. for 1 hour. Mean and 95% confidence intervals are shown (*p-values <0.05).