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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: J Periodontal Res. 2016 May 5;52(2):246–254. doi: 10.1111/jre.12388

Fig. 3. Red blood cells (RBC) and resolvin E1 (RvE1) reduce P. gingivalis–induced generation of reactive oxygen species (ROS) in neutrophils.

Fig. 3

Dihydroethidium (DHE)-labeled 5×105 neutrophils (PMN), from either healthy controls (n=6) (□) or subjects with localized aggressive periodontitis (LAgP) (n=6) (■) were suspended media containing 20% (v/v) AB serum and stimulated with 1×107 Porphyromonas gingivalis (P.g.) for 1 hour at 37°C in absence or presence of 3.6×107 autologous red blood cells (RBCs). All experiments were performed in triplicate. Half of the cultures received RvE1 (final concentration: 10nM). Each box represents the intracellular content of reactive oxygen species (ROS) of either healthy controls or patients with LAgP. Boxes indicate minimum and maximum with line at the mean. ROS are expressed as median fluorescence intensity (*p-values <0.05). RvE1 reduces intracellular ROS as do RBC; however, the additive effect is statistically significant only in subjects with LAgP (p<0.05), although there is a clear trend in controls as well (p=0.0652).