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Expression of COX-2 in IS-induced mouse MCs. MCs were treated with the indicated doses of IS (0, 250, and 500 μM) for 24 h and then COX-2 protein (a and b) and mRNA (c) and protein expressions were analyzed by Western blotting and qRT-PCR. A time course analysis of COX-2 mRNA expression following IS treatment at a dose of 500 μM was also examined (d). Values are means ± SD; n = 6 in each group; ∗ P < 0.05 versus control and ∗∗ P < 0.01 versus control.
