Table 1.

Primers for genes used for qPCR verification.

Trend	Unigene code	Annotated gene	Primer
0	Unigene27615_All	Brachyury	qBran-S: 5′ GCCAAAGAAAGACCAGAAGG 3′
			qBran-A: 5′ TCAGGCTAAGGCGATCACAA 3′
0	Unigene27517_All	Six3	qSix3-S: 5′ ATACAGGGTGAGGAAGAAGT 3′
			qSix3-A: 5′ TTATCTCCGCCTTGCTGTTG 3′
3	Unigene23318_All	Engrailed	qEng-S: 5′ TAGACAGAGCATCGCCTTTA 3′
			qEng-A: 5′ TTGTGATTTAACTGCCTGCT 3′
3	Unigene41009_All	Pax-7	qPax-S: 5′ GCGGAAACAGATGGGAAGCA 3′
			qPax-A: 5′ ACCGAATGACGGAAACGACT 3′
26	CL664.Crontig4_All	MAPK	qMAPK-S: 5′ TTTACTCCAAACAGCCCTAC 3′
			qMAPK-A: 5′ TTGCTATCTGGTCCACTTCA 3′
29	CL7953.Contig2_All	Notch	qNotch-S: 5′ CCAGCCACGGTATCCAAGTA 3′
			qNotch-A: 5′AGCCTCGAACAGAATATCCACT 3′
29	CL1306.Contig2_All	Wnt 1	qWnt1-S: 5′ TGATGCCTACGGTAAATACG 3′
			qWnt1-A: 5′ TAACCTTGAGGTGGGAGAAC 3′
29	Unigene27337_All	Lox2	qLox2-S: 5′ CTACCCGAGTTGAATGTGGG 3′
			qLox2-A: 5′ GAAAGTAAGACGGACGAGCC 3′