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. 2016 Nov 5;16:851. doi: 10.1186/s12885-016-2878-9

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — HMA affects cell morphology and mitochondria distribution. a TEM (transmission electron microscopy) analysis of diaminobenzidine (DAB) photo-oxidation in HCT-116 untreated cells and in cells treated with 20 μM HMA for 24 h. Red asterisk marks vesicles enclosing electron-dense regions within the cytoplasm; red arrowheads refer to vesicles containing intracellular debris. b Bright field microscopy images of HCT-116 cells untreated and treated with 20 μM HMA for 24 h; nuclei are marked with *. c Immunofluorescence analysis of mitochondrial HSP70 in untreated and HMA-treated (20 μM for 24 h) HCT-116 cells. Scale bar: 50 μm. A representative experiment out of three is shown