Fig. 5.

Effect of Olaparib on cell viability and autophagy. a Immunofluorescence detection of PAR (red) in HCT-116 cells treated for 24 h with 20 μM HMA without or with 10 μM Olaparib (OLAP). b MTT assay on samples as in (a) and in cells treated with OLAP only (10 μM for 24 h) or untreated; ***P < 0.001. c Immunofluorescence analysis of autophagy marker LC3 expression (green fluorescence) in HCT-116 cells under the above conditions; nuclei were counterstained with DAPI (blue fluorescence). Scale bar: 50 μm. A representative experiment out of three is shown