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. Author manuscript; available in PMC: 2017 Oct 20.
Published in final edited form as: Cell. 2016 Oct 20;167(3):670–683.e10. doi: 10.1016/j.cell.2016.09.023

Figure 6. Sca4 disrupts donor cell adherens junction complexes to relieve tension and promote spread.

Figure 6

(A) Competition co-IP assay between vinculin (HA-vinc 1-836aa), myc-α-catenin and FLAG-Sca4. (B) Model of Sca4 inhibition of vinculin function. In uninfected cells, vinculin and α-catenin promote junction stability via reciprocal actin-mediated pulling forces. Sca4 disrupts the vinculin:α-catenin interaction, thus reducing donor cell actomyosin tension and increasing membrane flexibility to improve spread efficiency. (C) Infectious focus size after 20 h blebbistatin (6.25 μm-25 μM) or DMSO (control) treatment. Mean ± SEM, unpaired T-test: ** p < 0.01 relative to WT + DMSO.