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. Author manuscript; available in PMC: 2016 Nov 6.
Published in final edited form as: Cell Rep. 2016 Oct 4;17(2):527–540. doi: 10.1016/j.celrep.2016.09.005

Figure 4.

Figure 4

Effect of Nat1 on mitochondrial dynamics in 3T3-L1 adipocytes. Nat1 knockdown increased mitochondrial fission/fragmentation. (A) Confocal imaging of mitochondrial fragmentation in 3T3-L1 adipocytes transduced with lenti virus for controls and sh Nat1 stained with MitoTracker Red CMXRos (100 nM) and analyzed for mitochondrial area, perimeter, circularity and interconnectivity. Western blot analysis of mitochondrial proteins for (B) Mitochondrial fission quantified by imageJ, values are mean ± SEM of 2 different experiments (*p ≤ 0.05). (C) Fusion (D) Mitophagy in isolated mitochondrial and cytosolic fractions from 3T3-L1 adipocytes transduced with lenti virus for controls and sh Nat1. (E) Mitochondrial markers in isolated mitochondrial fractions from WAT of wild type and Nat1 deficient mice (n = 4).