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. 2016 Nov 7;7:1772. doi: 10.3389/fmicb.2016.01772

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Copyright © 2016 Pisa, Alonso, Rábano, Horst and Carrasco.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of fungal chitin in brain sections of AD patients by immunohistochemistry. Entorhinal cortex (ERH) sections (5 μm) from 10 different AD patients were immunostained for 1 h with rabbit polyclonal anti-chitin antibodies (green) and mouse monoclonal anti-human α-tubulin antibodies (red). DAPI staining of nuclei appears in blue. Double immunostaining and confocal microscopy were carried out as indicated in the section “MATERIALS AND METHODS.” Scale bar: 10 μm.