FIGURE 4.

Detection of fungal enolase, β-tubulin, and chitin in different CNS regions of patient AD11. Immunohistochemistry analysis of CNS sections from patient AD11 were performed by confocal microscopy as detailed in the section of “MATERIALS AND METHODS.” LFC, lateral frontal cortex; CEC, cerebellar cortex; ERH, entorhinal cortex/hippocampus; CP, choroid plexus. Anti-enolase, anti-tubulin, and anti-chitin antibodies are shown in green. Double staining with mouse monoclonal anti-human α-tubulin inmunostaining for the anti-enolase antibody sample, human anti-neurofilament immunostaining for fungal anti-tubulin antibody sample, and anti-TauT100 staining for human anti-chitin antibody sample are shown in red. DAPI staining appears in blue. The different CNS sections are indicated in the panels. Scale bar: 10 μm.