Figure 6.

PYROXD1 Shows Nuclear Localization in Human Skin Fibroblasts and Transfected Cos-7 Cells

(A) Untransfected Cos-7 cells (top row) and Cos-7 cells transfected with human wild-type, p.Asn155Ser (N155S)-, or p.Gln372His (Q372H)-PYROXD1 expression constructs co-stained for PYROXD1 (green) and lamin A/C (red) shows enriched labeling for PYROXD1 within the nucleus, as well as labeling of cytoplasmic networks. For transfected Cos-7 cells (bottom three rows), asterisks demark untransfected cells within the field, with much lower levels of PyroxD1, that show very weak labeling under optimum capture conditions for transfected cells.

(B) Nuclear-cytoplasmic labeling is observed for endogenous PYROXD1 in primary human fibroblasts.