FIG 4.

ASFV-specific endpoint antibody titers. Antigen-specific endpoint titers of serum collected week 1 postboost were determined by ELISA. The endpoint dilution was determined to be the dilution at which the sample OD was higher than the OD of cognate prebleed plus 3 standard deviations. The lowest titer was 1:64 × 10³ against p72, and some of the highest titers were as high as 1:16 × 10⁶ against pp62. The ASFV-specific convalescent-phase serum was also titrated, and the titer against each antigen is indicated by a red star. Sera from T4 animals showed no reactivity above background to any of the antigens (see Fig. S3 in the supplemental material). The antigen-specific titers among the treatment groups were compared using ANOVA, followed by Tukey's multiple-comparison test.