FIG 5.

The Ad-ASFV multiantigen cocktail induced authentic ASFV-specific antibody responses. Indirect immunofluorescence antibody (IFA) assay and Western blotting using serum from week 1 postboost were used to confirm whether antibodies induced by the experimental Ad-ASFV multiantigen cocktail recognized ASFV-infected cells and ASFV-derived antigens. (A) Primary swine macrophages infected with the ASFV Georgia 2007/1 isolate and probed with individual, representative sera from six vaccinated and three control animals. ASFV-specific convalescent-phase serum was used as the positive (+ve) control, whereas normal pig serum was used as the negative control. The overall results are summarized in Table 2. (B) Western blots of lysates from Vero cells infected with the ASFV Georgia 2007/1 isolates, probed with sera from all animals. Lane 1, ASFV-specific convalescent-phase serum; lane 2, normal swine serum. The serum was also tested on mock-infected Vero cell lysates to check for background reactivity against host cell antigens (see Fig. S4 in the supplemental material).