Fig. 1.

Purification of granulocytes using magnetic microbeads. A, Graphical overview of granulocyte separation by phagocytosis of magnetic beads. Other hemocyte cell types and common contaminants in hemocoel perfusate are not involved in phagocytosis, enabling purification of a highly enriched granulocyte population. B, Light, Phase-contrast and Fluorescent microscopy were used to verify the uptake of magnetic beads by granulocytes, but not fat body, the main contaminant present in such samples. Cell stains and conserved Drosophila antibodies were used to further morphologically differentiate the two cell types.