Table I. Lysine methylation, identified on S. islandicus DNA/RNA-binding proteins by bottom-up approach with HCD and ETD fragmentation. Methylated residues are underlined and marked in bold.
| Uniprot accession | Protein description | Methylation sites | Sequence |
|---|---|---|---|
| M9UDF6 | Alba1 protein | 16* | SSGTPTPSN VVLIGKKPVM NYVLAALTLL |
| NQGVSEIVIK ARGRAISKAV DTVEIVRNRF | |||
| LPDKIEIKEI RVGSQVVTSQ DGRQSRVSTI EIAIRKK | |||
| M9U6G3 | Alba2 protein | 4 | TEKLNEIVV RKTKNVEDHV LDVIVLFNQG |
| IDEVILKGIG REISKAVDVY NSLKDRLGDG | |||
| IQLVNVQTGS EVRDRRRISY ILLRLKRVY | |||
| M9U8W4 | Chromatin protein Cren7 | 11, 16. 24, 31, 42 | SSGKKAVKV KTPAGKEAEL VPEKVWALAP |
| KGRKGVKIGL FKDPETGKYF RHKLPDDYPI | |||
| M9UD85 | 7-kDa DNA-binding protein (Sul7d1) | 5, 7, 28, 40, 49, 53, 61, 63.64 | TTVKFKYKG EEKQVDTSKI KKVWRVGKMI |
| SFTYDEGGGK TGRGAVSEKD APKELLQMLEKQKK | |||
| M9UII2 | 7-kDa DNA-binding protein (Sul7d2) | 28, 40, 49, 53, 61, 63.64 | VTVKFKYKG EDKEVDTSKI KKVWRVGKMI |
| SFTYDEGGGK TGRGAVSEKD APKELLQMLEKQKK |
* - only found in the in-gel digested 12 kDa SDS-PAGE band